ABSTRACT
Objectives: Obesity increases the risk of numerous chronic diseases. Obesity is classified clinically using body mass index [BMI], waist-to-hip ratio, and body fat percentage. The lipoprotein lipase [LPL] gene has been linked to lipoprotein metabolism and obesity. We performed a case-control study to determine the association between LPL gene polymorphisms and obesity-associated phenotypes such as insulin resistance [IR]
Methods: We examined the different LPL gene variants for association in 642 individuals segregated by BMI and IR. Genotyping of the LPL gene -93 and -53 promoter gene polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism
Results: A substantial association was observed for -93 gene polymorphism of the LPL gene with obesity, while -53 promoter gene polymorphism showed association with IR
Conclusions: We found a significant association between -93 and -53 promoter gene polymorphisms of the LPL gene with obesity and associated phenotypes in the studied population
ABSTRACT
Background: Obesity is a very common disorder resulting from an imbalance between food intake and energy expenditure, and it has a substantial impact on the development of chronic diseases. The aim of this study was to examine the association of INSIG2 [rs7566605] gene polymorphism with obesity and obesity associated phenotypes in North Indian subjects
Methods: The variants were investigated for association in 642 obese and non-obese individuals. The genotyping of INSIG2 [rs7566605] single nucleotide polymorphism was analyzed by the TaqMan allelic discrimination protocol
Results: A significant association was observed for INSIG2 [rs7566605] single nucleotide polymorphism with obesity and obesity-related phenotypes. Furthermore, a significant relationship was found between the rs7566605 and insulin, homeostasis model of assessment-insulin resistance, the percentage of body fat, fat mass, leptin, and adiponectin
Conclusion: The present study observed significant association between INSIG2 [rs7566605] single nucleotide polymorphism and obesity, as well as obesity associated phenotypes in North Indian population
Subject(s)
Humans , Male , Female , Adult , Membrane Proteins/genetics , Intracellular Signaling Peptides and Proteins/genetics , Polymorphism, Genetic , Phenotype , Polymorphism, Single Nucleotide , Body Mass Index , Insulin , Insulin ResistanceABSTRACT
Objectives: Obesity is a common disorder that has a significant impact on morbidity and mortality. Twin and adoption studies support the genetic influence on variation of obesity, and the estimates of the heritability of body mass index [BMI] is significantly high [30 to 70%]. Variants in the fat mass and obesity-associated [FTO] gene have been associated with obesity and obesity-related phenotypes in different populations. The aim of this study was to examine the association of FTO rs9939609 with obesity and related phenotypes in North Indian subjects
Methods: Gene variants were investigated for association with obesity in 309 obese and 333 non-obese patients. Genotyping of the FTO rs9939609 single nucleotide polymorphism [SNP] was analyzed using Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments. We also measured participants fasting glucose and insulin levels, lipid profile, percentage body fat, fat mass and fat free mass
Results: Waist to hip ratio, systolic blood pressure, diastolic blood pressure, percentage body fat, fat mass, insulin concentration, and homeostasis model assessment index [HOMA-Index] showed a significant difference between the study groups. Significant associations were found for FTO rs9939609 SNP with obesity and obesity-related phenotypes. The significant associations were observed between the rs9939609 SNP and blood pressure, fat mass, insulin, and HOMA-index under a different model. Conclusion: This study presents significant association between FTO rs9939609 and obesity defined by BMI and also established the strong association with several measures of obesity in North Indian population